Coding
Part:BBa_K3742001:Design
Designed by: Hanyi Zhang Group: iGEM21_BUCT-China (2021-09-29)
EC WS2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2532
Illegal EcoRI site found at 3171
Illegal PstI site found at 79
Illegal PstI site found at 604
Illegal PstI site found at 1003
Illegal PstI site found at 1045
Illegal PstI site found at 1108
Illegal PstI site found at 1773
Illegal PstI site found at 1833 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2532
Illegal EcoRI site found at 3171
Illegal PstI site found at 79
Illegal PstI site found at 604
Illegal PstI site found at 1003
Illegal PstI site found at 1045
Illegal PstI site found at 1108
Illegal PstI site found at 1773
Illegal PstI site found at 1833
Illegal NotI site found at 1430 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2532
Illegal EcoRI site found at 3171
Illegal BglII site found at 83
Illegal BglII site found at 947 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2532
Illegal EcoRI site found at 3171
Illegal PstI site found at 79
Illegal PstI site found at 604
Illegal PstI site found at 1003
Illegal PstI site found at 1045
Illegal PstI site found at 1108
Illegal PstI site found at 1773
Illegal PstI site found at 1833 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2532
Illegal EcoRI site found at 3171
Illegal PstI site found at 79
Illegal PstI site found at 604
Illegal PstI site found at 1003
Illegal PstI site found at 1045
Illegal PstI site found at 1108
Illegal PstI site found at 1773
Illegal PstI site found at 1833
Illegal NgoMIV site found at 1697
Illegal AgeI site found at 697
Illegal AgeI site found at 1306
Illegal AgeI site found at 1354
Illegal AgeI site found at 2220
Illegal AgeI site found at 2232
Illegal AgeI site found at 2490
Illegal AgeI site found at 2625 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
NCBI