Coding

Part:BBa_K3742001:Design

Designed by: Hanyi Zhang   Group: iGEM21_BUCT-China   (2021-09-29)


EC WS2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2532
    Illegal EcoRI site found at 3171
    Illegal PstI site found at 79
    Illegal PstI site found at 604
    Illegal PstI site found at 1003
    Illegal PstI site found at 1045
    Illegal PstI site found at 1108
    Illegal PstI site found at 1773
    Illegal PstI site found at 1833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2532
    Illegal EcoRI site found at 3171
    Illegal PstI site found at 79
    Illegal PstI site found at 604
    Illegal PstI site found at 1003
    Illegal PstI site found at 1045
    Illegal PstI site found at 1108
    Illegal PstI site found at 1773
    Illegal PstI site found at 1833
    Illegal NotI site found at 1430
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2532
    Illegal EcoRI site found at 3171
    Illegal BglII site found at 83
    Illegal BglII site found at 947
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2532
    Illegal EcoRI site found at 3171
    Illegal PstI site found at 79
    Illegal PstI site found at 604
    Illegal PstI site found at 1003
    Illegal PstI site found at 1045
    Illegal PstI site found at 1108
    Illegal PstI site found at 1773
    Illegal PstI site found at 1833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2532
    Illegal EcoRI site found at 3171
    Illegal PstI site found at 79
    Illegal PstI site found at 604
    Illegal PstI site found at 1003
    Illegal PstI site found at 1045
    Illegal PstI site found at 1108
    Illegal PstI site found at 1773
    Illegal PstI site found at 1833
    Illegal NgoMIV site found at 1697
    Illegal AgeI site found at 697
    Illegal AgeI site found at 1306
    Illegal AgeI site found at 1354
    Illegal AgeI site found at 2220
    Illegal AgeI site found at 2232
    Illegal AgeI site found at 2490
    Illegal AgeI site found at 2625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

NCBI

References